CRISPR: three new developments in the world of gene editing
A new variant for CRISPR
In a new study, researchers from Michigan Medicine at the University of Michigan, US, revealed that using a novel variant to repair DNA can improve both safety and effectiveness of CRISPR-Cas9 for genetic research.
According to the scientists, the safety and efficacy of CRISPR-Cas9 are what continue to hold the gene editing technique back from its full clinical potential.
To develop their variant, the researchers fused a minimal motif consisting of 36 amino acids to a gene-editing nuclease Streptococcus pyogenes Cas9 (spCas9). According to the team, the new meticulous integration Cas9 (miCas9) binds RAD51 through this fusion motif and enriches RAD51 at the target locus.